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4 6 diamidino 2 phenylindole dapi nuclear stain  (Vector Laboratories)


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    Vector Laboratories 4 6 diamidino 2 phenylindole dapi nuclear stain
    4 6 Diamidino 2 Phenylindole Dapi Nuclear Stain, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 21970 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4+6+diamidino+2+phenylindole+dapi+nuclear+stain/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/pmc11974669-96-15-21
    Average 98 stars, based on 21970 article reviews
    4 6 diamidino 2 phenylindole dapi nuclear stain - by Bioz Stars, 2026-09
    98/100 stars

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    Staining:

    Article Title: A macro-transection model of brain trauma for neuromaterial testing with functional electrophysiological readouts
    Article Snippet: .. After 3× PBS washes, coverslips were mounted on glass slides with Vectashield® mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain (Cat# H-1200, Vector Laboratories, Burlingame, CA,USA). .. Primary antibodies were rabbit anti-glial fibrillary acidic protein (GFAP) (1:500, Cat# Z0334, Santa Cruz Biotechnology, Heidelberg, Germany), mouse anti-β-tubulin (Tuj-1) (1:500, Cat# 801202, BioLegend, San Diego, CA, USA), goat antiionized calcium binding adaptor molecule 1 (Iba1) (1:500, Cat# ab5076, Abcam, Cambridge, UK), mouse anti-neuronal glial antigen2 (NG2) (1:500, Cat# ab275024, Abcam), rat antimyelin basic protein (MBP) (1:200) (Cat# 160223, BioRad, Hercules, CA, USA).

    Article Title: A macro-transection model of brain trauma for neuromaterial testing with functional electrophysiological readouts
    Article Snippet: .. After 3× PBS washes, coverslips were mounted on glass slides with Vectashield® mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain (Cat# H-1200, Vector Laboratories, Burlingame, CA, USA). .. Primary antibodies were rabbit anti-glial fibrillary acidic protein (GFAP) (1:500, Cat# Z0334, Santa Cruz Biotechnology, Heidelberg, Germany), mouse anti-β-tubulin (Tuj-1) (1:500, Cat# 801202, BioLegend, San Diego, CA, USA), goat anti-ionized calcium binding adaptor molecule 1 (Iba1) (1:500, Cat# ab5076, Abcam, Cambridge, UK), mouse anti-neuronal glial antigen2 (NG2) (1:500, Cat# ab275024, Abcam), rat anti-myelin basic protein (MBP) (1:200) (Cat# 160223, BioRad, Hercules, CA, USA).

    Article Title: Intracellular targeting of annexin A2 inhibits tumor cell adhesion, migration, and in vivo grafting.
    Article Snippet: KS1767, KRIB, B16F10 and LLC cells (ATCC) were maintained in Dulbecco’s modified Eagle’s Minimum Essential Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), vitamins, non-essential amino acids, penicillin/streptomycin, and L-glutamine (Gibco) at 37 °C in a 5% CO2 humidified incubator. .. The 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain was from Vector Laboratories. .. Human recombinant annexin A1, annexin A2, annexin A4 and annexin A5 were from Amprox; fibronectin and vitronectin were from R&D Systems; type I collagen I was from ScienCell Research Laboratories.

    Article Title: Tamoxifen Ameliorates Peritoneal Membrane Damage by Blocking Mesothelial to Mesenchymal Transition in Peritoneal Dialysis
    Article Snippet: .. After another washing process, the preparations were mounted with a 4,6-diamidino-2-phenylindole (DAPI) nuclear stain (Vectashield; Vector Laboratories). ..

    Article Title: Rapamycin Protects from Type-I Peritoneal Membrane Failure Inhibiting the Angiogenesis, Lymphangiogenesis, and Endo-MT
    Article Snippet: .. After another washing process, the preparations were mounted with a 4,6-diamidino-2-phenylindole (DAPI) nuclear stain (Vectashield; Vector Laboratories). ..

    Article Title: Immunohistochemical and Molecular Investigations Show Alteration in the Inflammatory Profile of Multiple System Atrophy Brain
    Article Snippet: .. Multi-immunofluorescence was performed using isotype specific antirabbit IgG, antimouse IgG, or antigoat IgG secondary antibodies conjugated with either Alexa 488 or 594 fluorescent dyes (1:400) (Life Technologies, Paisley, UK) followed by quenching of autofluorescence with 0.1% Sudan black/70% ethanol (Sigma-Aldrich, Dorset, UK) solution for 10 minutes and mounting with glass coverslips using VECTAshield mounting media with 4’,6-diamidino-2-phenylindole (DAPI) nuclear stain (Vector Laboratories, Peterborough, UK). .. Images were visualized using fluorescence microscopy (Leica DM5500 B).

    Article Title: Assessment of necroptosis in the retina in a repeated primary ocular blast injury mouse model
    Article Snippet: .. Tissue sections were washed 3 × 5 min in PBS then mounted in Vectashield mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain (Vector Laboratories, Peterborough, UK). ..

    Article Title: Osteoblast response to disordered nanotopography
    Article Snippet: A secondary biotinylated anti-mouse antibody (Vector Laboratories) was added [1:50] in 1% (w/v) BSA/PBS and the samples were incubated at 37°C for 1 h. The samples were washed as before and incubated with fluorescein isothiocyanate (FITC)-conjugated streptavidin (Vector Laboratories) [1:50] in 1% (w/v) BSA/PBS at 4°C for 30 min. .. Finally, the samples were washed again and mounted using VECTASHIELD mountant with 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain (Vector Laboratories). .. Protein intensity visualisation was performed by a fluorescence microscope (Zeiss Axiovert 200M, 10× magnification, numerical aperture (NA): 0.5).



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    ( A ) Cx43 stain of endomyocardial biopsies from ACM patients. Black arrows: Cx43 localization. Scale bar: 20 μm ( B ) Cx43 protein expression, quantification for the expression levels and localization in hiPSC-CM. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as loading control. ***, P < 0.001. One-way ANOVA with Tukey’s comparison test. Data from three clones. Sarcomeric alpha actinin (SαACT, green) denotes cardiomyocytes. Nuclear stain <t>viewed</t> <t>via</t> <t>4',6-diamidino-2-phenylindole</t> (DAPI) counterstain (blue). White arrows indicate Cx43 stain. Scale bar: 5μm. ( C ) Upper: Hematoxylin and eosin stain of endomyocardial biopsies. Scale bar: 100 μm Yellow arrowhead denotes cardiomyocyte loss and replacement fibrosis. Lower: Transmission electron microscopy analyses of hiPSC-CM. Yellow arrowhead denotes desmosome. Scale bar 500 nm. ( D-E ) Desmosomal protein expression and localization in hiPSC-CM. Sarcomeric alpha-actinin (SαACT, green) stain denotes cardiomyocytes. Nuclear stain is denoted by DAPI (blue). White arrows denote plakoglobin (JUP) stain. Beta-actin is used as a loading control. Scale bar 5μm. ( F ) Quantification of desmosomal gene expression in hiPSC-CM from desmoglein-2 mutant ( DSG2 -mut) and plakophilin-2 mutant ( PKP2 -mut) cardiomyocytes compared with Non-ACM. n = 3 for each gene, *, P < 0.05; **, P < 0.01; ***, P < 0.001 two-way ANOVA with Tukey’s multiple comparison test. ( G ) Representative electrical (field potential) traces from hiPSC-CM at baseline and under isoproterenol (ISO), 1 μM stimulation. Scale bar for electrical trace: vertical=amplitude of field potential (μV); horizontal=time (1 s). ( H ) Quantification of rhythm irregularity based on hiPSC-CM electrical index (field potential) at baseline and under ISO stimulation. **, P < 0.01; ***, P< 0.001. NS: Not significant. Non-ACM (n=10), DSG2 -mut (n=8), PKP2 -mut (n=9), two-way ANOVA with Sidak’s multiple comparison test (comparing ISO vs Basal); two-way ANOVA with Dunnett's multiple comparison test (comparing difference within different group).
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    ( A ) Cx43 stain of endomyocardial biopsies from ACM patients. Black arrows: Cx43 localization. Scale bar: 20 μm ( B ) Cx43 protein expression, quantification for the expression levels and localization in hiPSC-CM. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as loading control. ***, P < 0.001. One-way ANOVA with Tukey’s comparison test. Data from three clones. Sarcomeric alpha actinin (SαACT, green) denotes cardiomyocytes. Nuclear stain <t>viewed</t> <t>via</t> <t>4',6-diamidino-2-phenylindole</t> (DAPI) counterstain (blue). White arrows indicate Cx43 stain. Scale bar: 5μm. ( C ) Upper: Hematoxylin and eosin stain of endomyocardial biopsies. Scale bar: 100 μm Yellow arrowhead denotes cardiomyocyte loss and replacement fibrosis. Lower: Transmission electron microscopy analyses of hiPSC-CM. Yellow arrowhead denotes desmosome. Scale bar 500 nm. ( D-E ) Desmosomal protein expression and localization in hiPSC-CM. Sarcomeric alpha-actinin (SαACT, green) stain denotes cardiomyocytes. Nuclear stain is denoted by DAPI (blue). White arrows denote plakoglobin (JUP) stain. Beta-actin is used as a loading control. Scale bar 5μm. ( F ) Quantification of desmosomal gene expression in hiPSC-CM from desmoglein-2 mutant ( DSG2 -mut) and plakophilin-2 mutant ( PKP2 -mut) cardiomyocytes compared with Non-ACM. n = 3 for each gene, *, P < 0.05; **, P < 0.01; ***, P < 0.001 two-way ANOVA with Tukey’s multiple comparison test. ( G ) Representative electrical (field potential) traces from hiPSC-CM at baseline and under isoproterenol (ISO), 1 μM stimulation. Scale bar for electrical trace: vertical=amplitude of field potential (μV); horizontal=time (1 s). ( H ) Quantification of rhythm irregularity based on hiPSC-CM electrical index (field potential) at baseline and under ISO stimulation. **, P < 0.01; ***, P< 0.001. NS: Not significant. Non-ACM (n=10), DSG2 -mut (n=8), PKP2 -mut (n=9), two-way ANOVA with Sidak’s multiple comparison test (comparing ISO vs Basal); two-way ANOVA with Dunnett's multiple comparison test (comparing difference within different group).
    4 6 Diamidino 2 Phenylindole Dapi Nuclear Stain, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4+6+diamidino+2+phenylindole+dapi+nuclear+stain/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/pmc11974669-96-15-21
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    ( A ) Cx43 stain of endomyocardial biopsies from ACM patients. Black arrows: Cx43 localization. Scale bar: 20 μm ( B ) Cx43 protein expression, quantification for the expression levels and localization in hiPSC-CM. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as loading control. ***, P < 0.001. One-way ANOVA with Tukey’s comparison test. Data from three clones. Sarcomeric alpha actinin (SαACT, green) denotes cardiomyocytes. Nuclear stain <t>viewed</t> <t>via</t> <t>4',6-diamidino-2-phenylindole</t> (DAPI) counterstain (blue). White arrows indicate Cx43 stain. Scale bar: 5μm. ( C ) Upper: Hematoxylin and eosin stain of endomyocardial biopsies. Scale bar: 100 μm Yellow arrowhead denotes cardiomyocyte loss and replacement fibrosis. Lower: Transmission electron microscopy analyses of hiPSC-CM. Yellow arrowhead denotes desmosome. Scale bar 500 nm. ( D-E ) Desmosomal protein expression and localization in hiPSC-CM. Sarcomeric alpha-actinin (SαACT, green) stain denotes cardiomyocytes. Nuclear stain is denoted by DAPI (blue). White arrows denote plakoglobin (JUP) stain. Beta-actin is used as a loading control. Scale bar 5μm. ( F ) Quantification of desmosomal gene expression in hiPSC-CM from desmoglein-2 mutant ( DSG2 -mut) and plakophilin-2 mutant ( PKP2 -mut) cardiomyocytes compared with Non-ACM. n = 3 for each gene, *, P < 0.05; **, P < 0.01; ***, P < 0.001 two-way ANOVA with Tukey’s multiple comparison test. ( G ) Representative electrical (field potential) traces from hiPSC-CM at baseline and under isoproterenol (ISO), 1 μM stimulation. Scale bar for electrical trace: vertical=amplitude of field potential (μV); horizontal=time (1 s). ( H ) Quantification of rhythm irregularity based on hiPSC-CM electrical index (field potential) at baseline and under ISO stimulation. **, P < 0.01; ***, P< 0.001. NS: Not significant. Non-ACM (n=10), DSG2 -mut (n=8), PKP2 -mut (n=9), two-way ANOVA with Sidak’s multiple comparison test (comparing ISO vs Basal); two-way ANOVA with Dunnett's multiple comparison test (comparing difference within different group).
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    ( A ) Cx43 stain of endomyocardial biopsies from ACM patients. Black arrows: Cx43 localization. Scale bar: 20 μm ( B ) Cx43 protein expression, quantification for the expression levels and localization in hiPSC-CM. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as loading control. ***, P < 0.001. One-way ANOVA with Tukey’s comparison test. Data from three clones. Sarcomeric alpha actinin (SαACT, green) denotes cardiomyocytes. Nuclear stain <t>viewed</t> <t>via</t> <t>4',6-diamidino-2-phenylindole</t> (DAPI) counterstain (blue). White arrows indicate Cx43 stain. Scale bar: 5μm. ( C ) Upper: Hematoxylin and eosin stain of endomyocardial biopsies. Scale bar: 100 μm Yellow arrowhead denotes cardiomyocyte loss and replacement fibrosis. Lower: Transmission electron microscopy analyses of hiPSC-CM. Yellow arrowhead denotes desmosome. Scale bar 500 nm. ( D-E ) Desmosomal protein expression and localization in hiPSC-CM. Sarcomeric alpha-actinin (SαACT, green) stain denotes cardiomyocytes. Nuclear stain is denoted by DAPI (blue). White arrows denote plakoglobin (JUP) stain. Beta-actin is used as a loading control. Scale bar 5μm. ( F ) Quantification of desmosomal gene expression in hiPSC-CM from desmoglein-2 mutant ( DSG2 -mut) and plakophilin-2 mutant ( PKP2 -mut) cardiomyocytes compared with Non-ACM. n = 3 for each gene, *, P < 0.05; **, P < 0.01; ***, P < 0.001 two-way ANOVA with Tukey’s multiple comparison test. ( G ) Representative electrical (field potential) traces from hiPSC-CM at baseline and under isoproterenol (ISO), 1 μM stimulation. Scale bar for electrical trace: vertical=amplitude of field potential (μV); horizontal=time (1 s). ( H ) Quantification of rhythm irregularity based on hiPSC-CM electrical index (field potential) at baseline and under ISO stimulation. **, P < 0.01; ***, P< 0.001. NS: Not significant. Non-ACM (n=10), DSG2 -mut (n=8), PKP2 -mut (n=9), two-way ANOVA with Sidak’s multiple comparison test (comparing ISO vs Basal); two-way ANOVA with Dunnett's multiple comparison test (comparing difference within different group).
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    ( A ) Cx43 stain of endomyocardial biopsies from ACM patients. Black arrows: Cx43 localization. Scale bar: 20 μm ( B ) Cx43 protein expression, quantification for the expression levels and localization in hiPSC-CM. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as loading control. ***, P < 0.001. One-way ANOVA with Tukey’s comparison test. Data from three clones. Sarcomeric alpha actinin (SαACT, green) denotes cardiomyocytes. Nuclear stain <t>viewed</t> <t>via</t> <t>4',6-diamidino-2-phenylindole</t> (DAPI) counterstain (blue). White arrows indicate Cx43 stain. Scale bar: 5μm. ( C ) Upper: Hematoxylin and eosin stain of endomyocardial biopsies. Scale bar: 100 μm Yellow arrowhead denotes cardiomyocyte loss and replacement fibrosis. Lower: Transmission electron microscopy analyses of hiPSC-CM. Yellow arrowhead denotes desmosome. Scale bar 500 nm. ( D-E ) Desmosomal protein expression and localization in hiPSC-CM. Sarcomeric alpha-actinin (SαACT, green) stain denotes cardiomyocytes. Nuclear stain is denoted by DAPI (blue). White arrows denote plakoglobin (JUP) stain. Beta-actin is used as a loading control. Scale bar 5μm. ( F ) Quantification of desmosomal gene expression in hiPSC-CM from desmoglein-2 mutant ( DSG2 -mut) and plakophilin-2 mutant ( PKP2 -mut) cardiomyocytes compared with Non-ACM. n = 3 for each gene, *, P < 0.05; **, P < 0.01; ***, P < 0.001 two-way ANOVA with Tukey’s multiple comparison test. ( G ) Representative electrical (field potential) traces from hiPSC-CM at baseline and under isoproterenol (ISO), 1 μM stimulation. Scale bar for electrical trace: vertical=amplitude of field potential (μV); horizontal=time (1 s). ( H ) Quantification of rhythm irregularity based on hiPSC-CM electrical index (field potential) at baseline and under ISO stimulation. **, P < 0.01; ***, P< 0.001. NS: Not significant. Non-ACM (n=10), DSG2 -mut (n=8), PKP2 -mut (n=9), two-way ANOVA with Sidak’s multiple comparison test (comparing ISO vs Basal); two-way ANOVA with Dunnett's multiple comparison test (comparing difference within different group).
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    ( A ) Cx43 stain of endomyocardial biopsies from ACM patients. Black arrows: Cx43 localization. Scale bar: 20 μm ( B ) Cx43 protein expression, quantification for the expression levels and localization in hiPSC-CM. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as loading control. ***, P < 0.001. One-way ANOVA with Tukey’s comparison test. Data from three clones. Sarcomeric alpha actinin (SαACT, green) denotes cardiomyocytes. Nuclear stain <t>viewed</t> <t>via</t> <t>4',6-diamidino-2-phenylindole</t> (DAPI) counterstain (blue). White arrows indicate Cx43 stain. Scale bar: 5μm. ( C ) Upper: Hematoxylin and eosin stain of endomyocardial biopsies. Scale bar: 100 μm Yellow arrowhead denotes cardiomyocyte loss and replacement fibrosis. Lower: Transmission electron microscopy analyses of hiPSC-CM. Yellow arrowhead denotes desmosome. Scale bar 500 nm. ( D-E ) Desmosomal protein expression and localization in hiPSC-CM. Sarcomeric alpha-actinin (SαACT, green) stain denotes cardiomyocytes. Nuclear stain is denoted by DAPI (blue). White arrows denote plakoglobin (JUP) stain. Beta-actin is used as a loading control. Scale bar 5μm. ( F ) Quantification of desmosomal gene expression in hiPSC-CM from desmoglein-2 mutant ( DSG2 -mut) and plakophilin-2 mutant ( PKP2 -mut) cardiomyocytes compared with Non-ACM. n = 3 for each gene, *, P < 0.05; **, P < 0.01; ***, P < 0.001 two-way ANOVA with Tukey’s multiple comparison test. ( G ) Representative electrical (field potential) traces from hiPSC-CM at baseline and under isoproterenol (ISO), 1 μM stimulation. Scale bar for electrical trace: vertical=amplitude of field potential (μV); horizontal=time (1 s). ( H ) Quantification of rhythm irregularity based on hiPSC-CM electrical index (field potential) at baseline and under ISO stimulation. **, P < 0.01; ***, P< 0.001. NS: Not significant. Non-ACM (n=10), DSG2 -mut (n=8), PKP2 -mut (n=9), two-way ANOVA with Sidak’s multiple comparison test (comparing ISO vs Basal); two-way ANOVA with Dunnett's multiple comparison test (comparing difference within different group).
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    ( A ) Cx43 stain of endomyocardial biopsies from ACM patients. Black arrows: Cx43 localization. Scale bar: 20 μm ( B ) Cx43 protein expression, quantification for the expression levels and localization in hiPSC-CM. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as loading control. ***, P < 0.001. One-way ANOVA with Tukey’s comparison test. Data from three clones. Sarcomeric alpha actinin (SαACT, green) denotes cardiomyocytes. Nuclear stain <t>viewed</t> <t>via</t> <t>4',6-diamidino-2-phenylindole</t> (DAPI) counterstain (blue). White arrows indicate Cx43 stain. Scale bar: 5μm. ( C ) Upper: Hematoxylin and eosin stain of endomyocardial biopsies. Scale bar: 100 μm Yellow arrowhead denotes cardiomyocyte loss and replacement fibrosis. Lower: Transmission electron microscopy analyses of hiPSC-CM. Yellow arrowhead denotes desmosome. Scale bar 500 nm. ( D-E ) Desmosomal protein expression and localization in hiPSC-CM. Sarcomeric alpha-actinin (SαACT, green) stain denotes cardiomyocytes. Nuclear stain is denoted by DAPI (blue). White arrows denote plakoglobin (JUP) stain. Beta-actin is used as a loading control. Scale bar 5μm. ( F ) Quantification of desmosomal gene expression in hiPSC-CM from desmoglein-2 mutant ( DSG2 -mut) and plakophilin-2 mutant ( PKP2 -mut) cardiomyocytes compared with Non-ACM. n = 3 for each gene, *, P < 0.05; **, P < 0.01; ***, P < 0.001 two-way ANOVA with Tukey’s multiple comparison test. ( G ) Representative electrical (field potential) traces from hiPSC-CM at baseline and under isoproterenol (ISO), 1 μM stimulation. Scale bar for electrical trace: vertical=amplitude of field potential (μV); horizontal=time (1 s). ( H ) Quantification of rhythm irregularity based on hiPSC-CM electrical index (field potential) at baseline and under ISO stimulation. **, P < 0.01; ***, P< 0.001. NS: Not significant. Non-ACM (n=10), DSG2 -mut (n=8), PKP2 -mut (n=9), two-way ANOVA with Sidak’s multiple comparison test (comparing ISO vs Basal); two-way ANOVA with Dunnett's multiple comparison test (comparing difference within different group).
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    ( A ) Cx43 stain of endomyocardial biopsies from ACM patients. Black arrows: Cx43 localization. Scale bar: 20 μm ( B ) Cx43 protein expression, quantification for the expression levels and localization in hiPSC-CM. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as loading control. ***, P < 0.001. One-way ANOVA with Tukey’s comparison test. Data from three clones. Sarcomeric alpha actinin (SαACT, green) denotes cardiomyocytes. Nuclear stain viewed via 4',6-diamidino-2-phenylindole (DAPI) counterstain (blue). White arrows indicate Cx43 stain. Scale bar: 5μm. ( C ) Upper: Hematoxylin and eosin stain of endomyocardial biopsies. Scale bar: 100 μm Yellow arrowhead denotes cardiomyocyte loss and replacement fibrosis. Lower: Transmission electron microscopy analyses of hiPSC-CM. Yellow arrowhead denotes desmosome. Scale bar 500 nm. ( D-E ) Desmosomal protein expression and localization in hiPSC-CM. Sarcomeric alpha-actinin (SαACT, green) stain denotes cardiomyocytes. Nuclear stain is denoted by DAPI (blue). White arrows denote plakoglobin (JUP) stain. Beta-actin is used as a loading control. Scale bar 5μm. ( F ) Quantification of desmosomal gene expression in hiPSC-CM from desmoglein-2 mutant ( DSG2 -mut) and plakophilin-2 mutant ( PKP2 -mut) cardiomyocytes compared with Non-ACM. n = 3 for each gene, *, P < 0.05; **, P < 0.01; ***, P < 0.001 two-way ANOVA with Tukey’s multiple comparison test. ( G ) Representative electrical (field potential) traces from hiPSC-CM at baseline and under isoproterenol (ISO), 1 μM stimulation. Scale bar for electrical trace: vertical=amplitude of field potential (μV); horizontal=time (1 s). ( H ) Quantification of rhythm irregularity based on hiPSC-CM electrical index (field potential) at baseline and under ISO stimulation. **, P < 0.01; ***, P< 0.001. NS: Not significant. Non-ACM (n=10), DSG2 -mut (n=8), PKP2 -mut (n=9), two-way ANOVA with Sidak’s multiple comparison test (comparing ISO vs Basal); two-way ANOVA with Dunnett's multiple comparison test (comparing difference within different group).

    Journal: Circulation. Heart failure

    Article Title: Connexin-43 Restoration Alleviates Desmosomal Arrhythmogenic Cardiomyopathy

    doi: 10.1161/CIRCHEARTFAILURE.125.013801

    Figure Lengend Snippet: ( A ) Cx43 stain of endomyocardial biopsies from ACM patients. Black arrows: Cx43 localization. Scale bar: 20 μm ( B ) Cx43 protein expression, quantification for the expression levels and localization in hiPSC-CM. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as loading control. ***, P < 0.001. One-way ANOVA with Tukey’s comparison test. Data from three clones. Sarcomeric alpha actinin (SαACT, green) denotes cardiomyocytes. Nuclear stain viewed via 4',6-diamidino-2-phenylindole (DAPI) counterstain (blue). White arrows indicate Cx43 stain. Scale bar: 5μm. ( C ) Upper: Hematoxylin and eosin stain of endomyocardial biopsies. Scale bar: 100 μm Yellow arrowhead denotes cardiomyocyte loss and replacement fibrosis. Lower: Transmission electron microscopy analyses of hiPSC-CM. Yellow arrowhead denotes desmosome. Scale bar 500 nm. ( D-E ) Desmosomal protein expression and localization in hiPSC-CM. Sarcomeric alpha-actinin (SαACT, green) stain denotes cardiomyocytes. Nuclear stain is denoted by DAPI (blue). White arrows denote plakoglobin (JUP) stain. Beta-actin is used as a loading control. Scale bar 5μm. ( F ) Quantification of desmosomal gene expression in hiPSC-CM from desmoglein-2 mutant ( DSG2 -mut) and plakophilin-2 mutant ( PKP2 -mut) cardiomyocytes compared with Non-ACM. n = 3 for each gene, *, P < 0.05; **, P < 0.01; ***, P < 0.001 two-way ANOVA with Tukey’s multiple comparison test. ( G ) Representative electrical (field potential) traces from hiPSC-CM at baseline and under isoproterenol (ISO), 1 μM stimulation. Scale bar for electrical trace: vertical=amplitude of field potential (μV); horizontal=time (1 s). ( H ) Quantification of rhythm irregularity based on hiPSC-CM electrical index (field potential) at baseline and under ISO stimulation. **, P < 0.01; ***, P< 0.001. NS: Not significant. Non-ACM (n=10), DSG2 -mut (n=8), PKP2 -mut (n=9), two-way ANOVA with Sidak’s multiple comparison test (comparing ISO vs Basal); two-way ANOVA with Dunnett's multiple comparison test (comparing difference within different group).

    Article Snippet: Fluorescent-labeled secondary antibodies (1:400, Invitrogen) and Hoechst 33342 or 4',6-diamidino-2-phenylindole (DAPI) nuclear stain were used as indicated, followed by imaging using confocal microscopy (Olympus FV1000) or Keyence (UCSD microscopy Core, NS047101) and analyzed by NIH ImageJ software.

    Techniques: Derivative Assay, Functional Assay, Staining, Expressing, Control, Comparison, Clone Assay, H&E Stain, Transmission Assay, Electron Microscopy, Gene Expression, Mutagenesis